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CU-CPT22

SKU: orb1308422

Description

CU-CPT22 is a first-in-class small molecule probe that selectively inhibits the TLR1/TLR2 heterodimer by binding at its interface, competitively blocking agonist binding. It is a valuable tool for studying TLR1/2 signaling in both cellular and animal models of inflammation and infection.

Research Area

Immunology & Inflammation

Images & Validation

Key Properties

CAS Number1416324-85-0
MW362.37
Purity99.80% (May vary between batches)
FormulaC19H22O7
SMILESCCCCCCOC(=O)c1cc(O)c(=O)c2c(O)c(O)c(OC)cc2c1
TargetTLR
SolubilityDMSO:125 mg/mL (344.95 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (11.04 mM)

Bioactivity

Target IC50
TLR2/1:(ki)0.41 μM
In Vitro
A novel compound (CU-CPT22) that can compete with the synthetic triacylated lipoprotein (Pam3CSK4) binding to TLR1/2 with high inhibitory activity and specificity.CU-CPT22 is a toll-like inhibitor of receptor 1 and 2 (TLR1/2) ( IC50: 0.58±0.09 μM). CU-CPT22 is found to have no significant cytotoxicity at various concentrations up to 100 μM in RAW 264.7 cells. It is showed that CU-CPT22 is able to compete with Pam3CSK4 for binding to TLR1/2 (Ki: 0.41±0.07 μM). Which is consistent with its potency observed in the whole cell assay. Increasing the concentration of CU-CPT22 to 6 μM decreases the anisotropy to background levels. It is found that CU-CPT22 inhibits TLR1/2 signaling without affecting other TLRs, showing it is highly selective in intact cells. The result shows that CU-CPT22 can inhibit about 60% of TNF-αand 95% of IL-1β at 8 μM.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TLR2/1, TLR1/2, TLR, Toll-like Receptor (TLR), CUCPT22, CU-CPT22, CU-CPT-22, CU CPT22, Inhibitor, inhibit

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Quality Guarantee

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Key Properties

No computed properties available.

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Protocol Information

CU-CPT22 (orb1308422)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
¥ 1,170.00
2 mg
¥ 1,430.00
5 mg
¥ 1,950.00
1 ml x 10 mM (in DMSO)
¥ 2,080.00
10 mg
¥ 2,860.00
25 mg
¥ 4,810.00
50 mg
¥ 7,020.00
100 mg
¥ 9,880.00
200 mg
¥ 13,390.00
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