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CU-T12-9

SKU: orb1308425

Description

CU-T12-9 is a potent and selective TLR1/2 heterodimer agonist (EC50 = 52.9 nM) that activates the NF-κB pathway, inducing proinflammatory cytokines. This activity makes it a valuable research tool for studying innate/adaptive immunity in models of infection, vaccination, and inflammatory diseases.

Research Area

Immunology & Inflammation

Images & Validation

Key Properties

CAS Number1821387-73-8
MW362.31
Purity99.93%
FormulaC17H13F3N4O2
SMILESCNc1ccc(cc1-n1cnc(c1)-c1ccc(cc1)C(F)(F)F)[N+]([O-])=O
TargetTLR
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (13.8 mM);DMSO:250 mg/mL (690.02 mM)

Bioactivity

Target IC50
TLR2:52.9 nM (EC50)(in HEK-Blue cells)|TLR1:52.9 nM (EC50)(in HEK-Blue cells)
In Vitro
CU-T12-9 directly targets TLR1/2 to initiate downstream signaling. It specifically induces TLR1/2 activation, which can be blocked by either the anti-hTLR1 or anti-hTLR2 antibody, but not the anti-hTLR6 antibody. By binding to both TLR1 and TLR2, CU-T12-9 facilitates TLR1/2 heterodimer formation, activating downstream signaling. Fluorescence anisotropy assays revealed competitive binding to the TLR1/2 complex between CU-T12-9 and Pam3CSK4, with an IC50 of 54.4 nM. CU-T12-9 signals through nuclear factor κB (NF-κB) and elevates downstream effectors tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), and inducible nitric oxide synthase (iNOS).

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

inhibit, infectious, inflammatory, influenza, pancreatic, obesity, Inhibitor, breast, diseases, CU T12 9, CUT129, CU-T-12-9, CU-T12-9, asthma, bladder, carcinoma, cancer, TLR, TLR1, TLR2, TLR1/2, Toll-like Receptor (TLR), specific

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  • CU-T12-9 [orb1219378]

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    C17H13F3N4O2

    500 mg, 5 mg, 10 mg, 25 mg, 100 mg, 1 g, 2 mg, 50 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Key Properties

No computed properties available.

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Protocol Information

CU-T12-9 (orb1308425)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
¥ 1,170.00
5 mg
¥ 2,080.00
1 ml x 10 mM (in DMSO)
¥ 2,210.00
10 mg
¥ 2,860.00
25 mg
¥ 4,420.00
50 mg
¥ 6,500.00
100 mg
¥ 9,100.00
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