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Cucurbitacin E

SKU: orb1301289

Description

Cucurbitacin E

Research Area

Cell Biology

Images & Validation

Key Properties

CAS Number18444-66-1
MW556.69
Purity98.88%
FormulaC32H44O8
SMILESCC(=O)OC(C)(C)\C=C\C(=O)[C@](C)(O)[C@H]1[C@H](O)C[C@@]2(C)[C@@H]3CC=C4[C@@H](C=C(O)C(=O)C4(C)C)[C@]3(C)C(=O)C[C@]12C
TargetCDK,Autophagy
Solubility10% DMSO+90% Corn Oil:2.5 mg/mL (4.49 mM);DMSO:55 mg/mL (98.8 mM)

Bioactivity

In Vitro
The anti-proliferation and cell cycle G2/M arrest induced by Cucurbitacin E (CuE) in CRC cells. MPM-2 flow cytometry tests show that CuE-treated cells accumulated in metaphase CuE 2.5-7.5 μM). CuE produced G2/M arrest as well a the downregulation of CDC2 and cyclin B1 expression and dissociation. Both effects increased proportionally wit the dose of CuE; however the Inhibition of proliferation, arrest of mitosis, production of Reactive oxygen species (ROS), and loss of mitochondrial membrane potential (ΔΨm) were found to be dependent o the quantity of CuE used to trea the cancer cells. In addition, cell cycle arrest in treated cells coincided wit the activation o the gene GADD45(α, β, γ). Incubation with CuE resulted in the binding of GADD45γ to CDC2, which suggests tha the delay in CuE-induced mitosis is regulated b the overexpression of GADD45γ. CuE may have antitumor activities in established CR.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
DisclaimerFor research use only

Alternative Names

NF-κB, p65, IL Receptor, JAK, inhibit, Inhibitor, ERK, Autophagy, Caspase, Cyclin dependent kinase, Cucurbitacin E, CDK, CDK1/cyclinB1, p21, p38 MAPK, TNF-α, ROS, STAT, α-Elaterin, α-Elaterine, VEGFR

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Key Properties

No computed properties available.

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Cucurbitacin E (orb1301289)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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1 mg
¥ 1,300.00
5 mg
¥ 2,080.00
1 ml x 10 mM (in DMSO)
¥ 2,470.00
10 mg
¥ 2,990.00
25 mg
¥ 4,680.00
50 mg
¥ 6,630.00
100 mg
¥ 9,360.00
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