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Cyclin B1 Mouse Monoclonal Antibody
SKU: orb499772
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:500-1000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 7D1 |
| Immunogen | KLH conjugated synthetic peptide derived from human Cyclin B1 |
| Target | CCNB1 |
| Molecular Weight | 48 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
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Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (ascites of orb499772 7D1) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (orb499772 7D1) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Quick Database Links
Gene Symbol
CCNB1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Cyclin B1 Mouse Monoclonal Antibody (orb499772)
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