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Cyclin B1 Antibody
SKU: orb1294340
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100–1:200 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
| Target | Cyclin B1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Cyclin B1 antibody - at 1/500 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294340 overnight at 4°C.
Huh-7 cell were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294340 anti-Cyclin B1 antibody (red) at 1:400 and 4°C.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling Cyclin B1 with orb1294340 at 1/100.
Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Cyclin B1 with orb1294340 at 1/100.
Quick Database Links
Gene Symbol
Cyclin B1
Documents Download
Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Cyclin B1 Antibody (orb1294340)
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