Cytokeratin 8 rabbit pAb Antibody

SKU: orb765010

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Description

Rabbit polyclonal antibody to Cytokeratin 8

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Keratin 8. AA range:401-450
Molecular Weight	53
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-KRT8 antibody, anti-CYK8 antibody, anti-Keratin antibody, anti- type II cytoskeletal 8 antibody, anti-Cytokeratin-8 antibody, anti-CK-8 antibody, anti-Keratin-8 antibody, anti-K8 antibody, anti-Type-II keratin Kb8 antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western Blot analysis of various cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of 293 cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of HeLa cells, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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