Denecimig Antibody

SKU: orb1942185

Description

Denecimig (Mim8) is a humanized bispecific IgG4κ antibody that mimics activated factor VIII function, promoting FIXa and FX assembly to enhance thrombin generation and hemostasis. It is applied in preclinical research for hemophilia A, including studies in relevant in vitro assays and in vivo bleeding models.

Research Area

Metabolism Research

Images & Validation

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Key Properties

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CAS Number	2488745-86-2
MW	145.47 kDa
Purity	95.00%
Target	Factor VIII,Factor Xa

Bioactivity

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In Vivo

In the toxicity study of Denecimig using monkeys as subjects, intravenous or subcutaneous administration (0.3–60 mg/kg/week) was employed. The Results showed that Denecimig (0.3–3 mg/kg/week, subcutaneous injection) did not induce significant clinical symptoms, excessive coagulation, or pathological changes. However, when the dose was increased to 6–20 mg/kg/week, 16% of the animals exhibited thrombosis-related pathological changes.

In Vitro

In a study of hemostatic activity in blood samples from hemophilia patients, both Thrombin Generation Assay (TGA) and Thromboelastography (TEG) were used for evaluation. TGA was performed using PPP-low reagent and PRP reagent. Whole blood samples were analyzed with the ROTEMdelta device for NATEM analysis, providing a comprehensive assessment of the coagulation status. The study also evaluated the effects of Denecimig when used in combination with recombinant FVIII (100 IU/dL), recombinant FVIIa (25 mM), and APCC (20 and 30 U/kg). The in vitro Results showed that Denecimig significantly enhanced thrombin generation in severe hemophilia A patients, with its procoagulant effect comparable to 100 IU/dL of FVIII. Further research found that when Denecimig was used in combination with rFVIIa (90 µg/kg), it did not induce a hypercoagulable state. However, when Denecimig was combined with APCC, it could significantly increase the risk of hypercoagulation .

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Mim8, Factor Xa, FactorXa

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Gene Symbol

Factor VIII,Factor Xa

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