Di-Methyl-Histone H3 (Lys27) Antibody

SKU: orb685607

Description

This Di-Methyl-Histone H3 (Lys27) Antibody is an unconjugated polyclonal product. It tagets HIST1H3A using a Peptide sequence around Di-Methylation site of lysine 27(A-R-K(di-methyl)-S-A) derived from Human Histone H3 as the immunogen. This antibody is suitable for ELISA, IHC, WB.

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution range	WB: 1: 500-1: 1000, IHC-P: 1: 50-1: 100
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Peptide sequence around Di-Methylation site of lysine 27(A-R-K(di-methyl)-S-A) derived from Human Histone H3.
Target	HIST1H3A
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy u
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Buffer/Preservatives	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer	For research use only

Alternative Names

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Anti-H3/a antibody, anti- H3/c antibody, anti- H3/d antibody, anti- H3/f antibody, anti- H3/h antibody

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Quality Guarantee

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Immunohistochemical staining of human breast carcinoma tissue using Di-Methyl-Histone H3 (Lys27) antibody

Western blot analysis of C6 and Hela cells using Di-Methyl-Histone H3 (Lys27) antibody

Quick Database Links

Gene Symbol

HIST1H3A

UniProt

P68431

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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