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DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb
SKU: orb1563253
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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IF, IP, WB |
|---|---|
| Dilution range | WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 5F6-6G6-6F9 |
| Immunogen | Synthetic Peptide of Histone H3 (Di Methyl Lys27) |
| Target | H3C1 |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Quality Guarantee
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Immunoprecipitation analysis of DiMethyl-Histone H3 (Lys27) (5F6) in Hela lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody.
Western blot analysis of DiMethyl-Histone H3 (Lys27) (5F6) in C6, 3T3, C2C12 lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody.
Immunocytochemistry analysis ofDiMethyl-Histone H3 (Lys27) in HeLa cells using DiMethyl-Histone H3 (Lys27) (5F6) antibody.
Western blot analysis of DiMethyl-Histone H3 (Lys27) (5F6) in Hela, rat heart, mouse heart, HUVEC, Jurkat lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody.
Quick Database Links
Gene Symbol
H3C1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb (orb1563253)
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