Donkey IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347995

Description

Donkey IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:10,000 - 1:40,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Donkey
Application Notes	Anti-Donkey IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Donkey IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Donkey IgG, and Donkey Serum.
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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goat anti-Donkey IgG Antibody alkaline phosphatase conjugation, goat anti-Donkey IgG alkaline phosphatase conjugated Antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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