You have no items in your shopping cart.
Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
SKU: orb347954
Description
Images & Validation
−Item 1 of 1
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Reactivity | Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Donkey |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Sheep IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins. |
| Conjugation | AP |
Storage & Handling
−| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Donkey Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Donkey Anti-Sheep IgG Antibody Alk phos Conjugation
Similar Products
−- Item 1 of 1
Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed [orb347741]
ELISA, IHC, WB
Rabbit
Sheep
Polyclonal
AP
1 mg 
F(ab')2 Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed [orb348390]
ELISA, IHC, WB
Sheep
Donkey
Polyclonal
AP
500 μgSheep IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed [orb347933]
ELISA, IHC, WB
Sheep
Rabbit
Polyclonal
AP
500 μg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
ELISA results of purified Polyclonal Donkey Anti-Sheep IgG Antibody Alkaline Phosphatase Conjugated tested against Sheep IgG. Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer and Substrate.
Quick Database Links
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed (orb347954)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days