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EBI3 Antibody
SKU: orb1238976
Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-EBI-3 antibody (orb1238976) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The immunogen is located within the last 50 amino acids of EBI3. |
| Target | EBI3 |
| Molecular Weight | Predicted: 25kDObserved: 30 kD (25kD + 2 N-Glycosylations) |
| Purification | EBI3 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | EBI3 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta
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Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane. Antibodies: EBI-3 orb1238976, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL, Lane 2: 2 µg/mL, Lane 3: 4 µg/mL.
Immunohistochemistry Validation of EBI-3 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded Human Spleen tissue using anti-EBI-3 antibody (orb1238976) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of EBI-3 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen tissue labeling EBI-3 with orb1238976 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
EBI3 Antibody (orb1238976)
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