EGFR Rabbit Polyclonal Antibody

SKU: orb584868

Description

Rabbit polyclonal antibody to EGFR

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 3

Tested Applications	WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Target	EGFR
Molecular Weight	69kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2

Quality Guarantee

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Lanes: Lane 1: 4 ug DRM fraction from MDA-MB-231, Primary Antibody dilution: 1:1000, Secondary Antibody: Donkey anti-rabbit IgG-HRP, Secondary Antibody dilution: 1:10000, Gene Name: EGFR. EGFR is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB231 cells.

Rabbit Anti-EGFR Antibody, Catalog Number: orb584868, Formalin Fixed Paraffin Embedded Tissue: Human Thyroid Gland Tissue, Observed Staining: Cytoplasm, Primary Antibody Concentration: 1:100, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-EGFR Antibody, Titration: 1.0 ug/ml, Positive Control: Placenta.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

RefSeq (Protein):NP_958439

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_958439

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Protocol Information

Western Blot (IB, immunoblot)

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