EGFR Mouse Monoclonal Antibody

SKU: orb500624

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Description

Research Area

Cancer Research, Cell Biology, Cell Differentiation, EGFR (Epidermal Growth Factor Receptor) Signaling, Epidermal Growth Factor, Receptor Tyrosne Kinases, SARS /COVID-19

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	13B2
Immunogen	KLH conjugated synthetic peptide derived from human EGFR
Target	EGFR
Molecular Weight	130 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EGFR; Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-EGFR (orb500624) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 170 kD, Observed band size: 180 kD.

Sample: Hela KO EGFR (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-EGFR (orb500624) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 170 kD.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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