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EGFR Mouse Monoclonal Antibody (Cy5)
SKU: orb969172
Description
Research Area
Cancer Research, Cell Biology, Cell Differentiation, EGFR (Epidermal Growth Factor Receptor) Signaling, Epidermal Growth Factor, Receptor Tyrosne Kinases, SARS /COVID-19
Images & Validation
−Item 1 of 1
| Tested Applications | IF |
|---|---|
| Dilution Range | IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 13B2 |
| Immunogen | KLH conjugated synthetic peptide derived from human EGFR |
| Target | EGFR |
| Purification | Affinity purified by Protein G |
| Conjugation | Cy5 |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EGFR; Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-EGFR Antibody, conjugated 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
EGFR
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Datasheet
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EGFR Mouse Monoclonal Antibody (Cy5) (orb969172)
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