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Featured
KO/KD
Validated
Validated
Description
Research Area
Cell Biology
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB=1:2000-20000, IHC-P=1:200-500, IHC-F=1:200-500, ICC/IF=1:100-500, IF=1:200-500, Flow-Cyt=1:100-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B5C0 |
| Immunogen | A synthesized peptide derived from human EGFR (1080-1110aa) |
| Target | EGFR |
| Molecular Weight | 170-175 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog); erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian); erb-b2 receptor tyrosine kinase 1
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Quick Database Links
Gene Symbol
EGFR
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry






























