Rabbit EGFR Recombinant Monoclonal Antibody

SKU: orb1784586

Description

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 7

Tested Applications	ICC, IHC, IP, WB
Dilution Range	Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR252L
Immunogen	Between 1160 and C-terminus
Target	EGFR
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

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Rabbit EGFR Recombinant Monoclonal Antibody

Detection of human EGFR by western blot. Samples: Whole cell lysate (10 µg) from GaMG, HeLa, OVCAR-8, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse EGFR by western blot. Samples: Whole cell lysate (10 µg) from AML12, NIH 3T3, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse EGFR (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human EGFR (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human EGFR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used for IP at 6 µl per reaction.

Detection of human EGFR by immunocytochemistry. Sample: FFPE section of A-431 cells. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

RefSeq (Protein):NP_958440.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_958440.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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Immunoprecipitation

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