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ELP2 Antibody (C-term)
SKU: orb34272
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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution range | IF/ICC: 1:100, WB: 1:1000, FACS: 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB18010 |
| Immunogen | Synthetic Peptide |
| Target | ELP2 |
| Molecular Weight | 92500 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Anti-Elongator complex protein 2 antibody, anti-ELP2 antibody, anti-STAT3-interacting protein antibody, anti-StIP1 antibody, anti-SHINC-2 antibody, anti-STATIP1 antibody, anti-ELP2 antibody
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Western blot analysis of ELP2 antibody (C-term) in NCI-H460 cell line lysates (35 ug/lane). ELP2 (arrow) was detected using the purified Pab.
Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ELP2 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Quick Database Links
Gene Symbol
ELP2
UniProt
RefSeq (Protein):NP_060725.1, NP_001229804.1, NP_001229805.1, NP_001229806.1, NP_001229807.1, NP_001229808.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein productDocuments Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELP2 Antibody (C-term) (orb34272)
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