ENaC alpha Antibody (HRP)

SKU: orb152719

Description

Rabbit polyclonal antibody against ENaC Alpha conjugated to HRP

Images & Validation

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Tested Applications	ICC, IF, IHC, IP, WB
Dilution range	WB (1:1000), ICC/IF (1:400); IHC (1:25)
Reactivity	Frog, Human, Mouse, Rat
Application Notes	1 µg/ml of SPC-403 was sufficient for detection of alpha-ENaC in 35 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced against a synthetic peptide mapping to the N-terminal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).
Target	ENaC alpha
Molecular Weight	85kDa
Purification	Protein A Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Alpha ENaC, Alpha ENaC 2, Alpha NaCH, Alpha-ENaC, Amiloride sensitive epithelial sodium channel alpha subunit, Amiloride sensitive sodium channel subunit alpha, Amiloride-sensitive sodium channel subunit alpha, ENaCa, ENaCalpha, Epithelial Na(+) channel subunit alpha, Epithelial Na+ channel subunit alpha, Epithelial Sodium Channel alpha, Epithelial Sodium Channel-α, FLJ21883, Nonvoltage gated sodium channel 1 subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN 1, SCNN1, SCNN1A, SCNNA_HUMAN, Sodium channel nonvoltage gated 1 alpha

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Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) ENaC Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

ENaC alpha

UniProt

Q6IRJ1

NCBI Gene ID

25122

RefSeq (Protein):NP_113736

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_113736

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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