ENaC alpha Antibody (HRP)

SKU: orb383813

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Description

Mouse monoclonal antibody against ENaC alpha conjugated to HRP.

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1:1000); IHC (1:150)
Reactivity	Mouse, Rat
Application Notes	A 1:1000 dilution of SMC-242 was sufficient for detection of ENaC alpha in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	1.4E+11
Immunogen	Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
Target	ENaC alpha
Molecular Weight	85kDa
Purification	Protein G Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Alpha ENaC, Alpha ENaC 2, Alpha NaCH, Alpha-ENaC, Amiloride sensitive epithelial sodium channel alpha subunit, Amiloride sensitive sodium channel subunit alpha, Amiloride-sensitive sodium channel subunit alpha, ENaCa, ENaCalpha, Epithelial Na(+) channel subunit alpha, Epithelial Na+ channel subunit alpha, Epithelial Sodium Channel alpha, Epithelial Sodium Channel-α, FLJ21883, Nonvoltage gated sodium channel 1 subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN 1, SCNN1, SCNN1A, SCNNA_HUMAN, Sodium channel nonvoltage gated 1 alpha

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Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10. Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.

Quick Database Links

Gene Symbol

ENaC alpha

UniProt

Q6IRJ1

NCBI Gene ID

25122

RefSeq (Protein):NP_113736

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_113736

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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