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Description
Research Area
Epigenetics, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IHC-P: 1:50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1203 of human eNOS (NP_000594.2). |
| Molecular Weight | 140kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti NOS3 antibody
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Immunofluorescence analysis of human placenta cells using eNOS Rabbit pAb (orb135993) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using eNOS antibody (orb135993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
El-Moselhy, Mohamed A. et al. Protective mechanisms of atorvastatin against doxorubicin-induced hepato-renal toxicity Biomed Pharmacother, 68, 101-110 (2014)
eNOS Rabbit pAb (orb135993)
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