EpCAM Antibody / Extracellular domain

SKU: orb606357

Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Canine, Feline, Human
Application Notes	Titering of the anti-EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	EGP40/1372
Immunogen	A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Immunofluorescent staining of FFPE human colorectal carcinoma with anti-EpCAM antibody (green) and Reddot nuclear stain (red).

Immunofluorescent staining of human MCF-7 cells with anti-EpCAM antibody (green, clone EGP40/1372) and Reddot nuclear stain (red).

Western blot testing of human 1) HEK293, 2) A431 and 3) HCT116 cell lysate with anti-EpCAM antibody (clone EGP40/1372). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

IHC testing of FFPE human hepatocellular carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human colorectal carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

Western blot testing of human lung lysate with anti-EpCAM antibody (clone EGP40/1372). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Flow cytometry testing of PFA-fixed human MCF7 cells with anti-EpCAM antibody (clone EGP40/1372); Red = isotype control, Blue = anti-EpCAM antibody.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using the anti-EpCAM antibody (clone EGP40/1372). These results demonstrate the foremost specificity of the EGP40/1372 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free anti-EpCAM antibody (clone EGP40/1372) as confirmation of integrity and purity.

IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.

Quick Database Links

UniProt

P16422

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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