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EpCAM/CD326 (Extracellular domain) Antibody
SKU: orb1294311
Description
Images & Validation
−Item 1 of 9
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:400 Immunohistochemistry (Paraffin) 1:100-1:150 Flow Cytometry 1:50-1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human EpCAM |
| Target | EpCAM / CD326 (Extracellular domain) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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FC, IF, IHC
Human
Mouse
Monoclonal
Unconjugated
500 μl
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Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.
Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.
Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: EpCAM antibody (extracellular domain) (orb1294311).
Cell: MCF-7 (DAPI: 1-300, Phalloidin: 1-300), EpCAM, (orb1294311): 1-200, Anti-rabbit 594: 1-500, NikonEpi-fluorescence (A-ken) 60x.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
EpCAM antibody, orb1294311, Cell: MCF-7.
Quick Database Links
Gene Symbol
EpCAM / CD326 (Extracellular domain)
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
EpCAM/CD326 (Extracellular domain) Antibody (orb1294311)
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