Fab Rabbit IgG (H&L) Antibody

SKU: orb348483

Description

Fab Rabbit IgG (H&L) antibody

Research Area

Epigenetics & Chromatin

Images & Validation

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Item 1 of 1

Tested Applications	DOT, ELISA, IHC, WB
Dilution Range	ELISA: 1:4,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Rabbit
Application Notes	Fab Anti-Rabbit IgG (H&L) Antibody has been tested by dot blot and SDS-PAGE and is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG Fab
Immunogen	Rabbit IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Goat Fab Anti-Rabbit IgG Antibody, Goat Fab Fragment Anti-Rabbit IgG Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

SDS PAGE of Fab Anti-RABBIT IgG (GOAT) Antibody. Lane 1: 1x Reduced, Fab Anti-RABBIT IgG Antibody. Lane 2: Molecular Weight Ladder. Lane 3: 1x Non-Reduced, Fab Anti-RABBIT IgG Antibody. 4-20% Lonza SDS-PAGE; Coomassie Stained.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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