F(ab')2 Goat IgG (H&L) Antibody Pre-Adsorbed

SKU: orb348122

Description

F(ab')2 Goat IgG (H&L) antibody

Images & Validation

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Item 1 of 2

Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Goat
Application Notes	F(ab')2 Anti-Goat IgG Antibody has been tested by SDS-PAGE and dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Goat IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Donkey F(ab')2 Anti-Goat IgG Antibody, Donkey Fab2 Anti-Goat IgG Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

F(ab')2 Goat IgG (H&L) Antibody Pre-Adsorbed

Dot Blot Results of F(ab')2 Donkey Anti-GOAT IgG [H&L] Antibody Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, Rt Serum Proteins. Goat IgG (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: F(ab')2 Dk-a-Gt IgG Mx8 at 1 µg/ml for 1hr at RT. Secondary Antibody: Goat Anti-Donkey HRP at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 mins at RT. Exposure: 1 sec.

SDS PAGE Results of F(ab')2 Anti-GOAT IgG (DONKEY) Antibody. Lane 1: Fab2 Dk-a-Gt IgG Mx8 Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Fab2 Dk-a-Gt IgG Mx8 Non-Reduced [1.0 µg]. 4-20% Gel, Coomassie Stained.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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