F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb348254

Description

F(ab')2 Mouse IgG (H&L) antibody (Peroxidase)

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:300:000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Reactivity	Mouse
Application Notes	F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Mouse IgG Antibody Peroxidase Conjugation, Goat Fab2 Anti-Mouse IgG HRP conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Western Blot of Goat anti-Fab2 Mouse IgG Antibody Peroxidase Conjugated Pre-Absorbed. Lane 1: Rabbit Serum. Lane 2: Rabbit Serum. Lane 3: Human Serum. Lane 4: Human Serum. Lane 5: Human IgG. Lane 6: Rat IgG. Lane 7: Bovine IgG. Lane 8: Horse IgG. Lane 9: Rabbit IgG. Lane 10: Mouse IgG. Load: Lane 1, 3 - 2 uL, Lane 2, 4 - 5 uL, Lanes 5 through 10 - 100 ng. Primary Antibody: none Secondary Antibody: Goat anti-Fab2 Mouse IgG Antibody Peroxidase Conjugated Pre-Absorbed at 1:5000 for 1 HR at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 160 kDa for Mouse IgG.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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