F(ab')2 Mouse IgG (H&L) Antibody Pre-Adsorbed

SKU: orb348275

Description

F(ab')2 MOUSE IgG (H&L) antibody

Images & Validation

−

Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:2,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:10,000
Reactivity	Mouse
Application Notes	F(ab')2 Mouse IgG (H&L) Antibody has been tested by ELISA and SDS-PAGE and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

−

Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.1 mg/mL
Disclaimer	For research use only

Alternative Names

−

Rabbit F(ab')2 Anti-Mouse IgG Antibody Pre-Adsorbed

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

F(ab')2 Mouse IgG (H&L) Antibody Pre-Adsorbed

SDS-PAGE of F(ab')2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Lane 1: Reduced F(ab')2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Lane 2: 5 µl OPAL Pre-stained Marker. Lane 3: Non-reduced F(ab')2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 75 kDa, Reduced at 25 kDa.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols