F(ab')2 Rat IgG Fc Antibody Pre-Adsorbed

SKU: orb348339

Description

F(ab')2 Rat IgG F(c) antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:3,000, WB: 1:2,000 - 1:5,000
Reactivity	Rat
Application Notes	F(ab')2 Anti-Rat IgG F(c) Antibody has been tested by SDS=PAGE and is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Anti-Rat IgG F(c) was produced by repeated immunization with Rat F(c) fragment in goat.
Purity	Anti-Rat F(c) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Rat IgG F(c) Antibody, Goat Fab2 Anti-Rat IgG Fc Antibody

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F(ab')2 Rat IgG Fc Antibody Pre-Adsorbed

SDS-PAGE results of Goat F(ab')2 Anti-Rat IgG F(c) Antibody. Lane 1: reduced Goat F(ab')2 anti-Rat. Lane 2: Opal Prestained Ladder. Lane 3: non-reduced Goat F(ab')2 anti-Rat. Load: 1.0ug. 4-20% Lonza SDS-PAGE; Coomassie Stained.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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