Fibroblasts, Mouse, mAb ER-TR7

SKU: orb1109119

Description

Mouse monoclonal antibody to Fibroblasts

Images & Validation

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Tested Applications	FC, IF, IHC-Fr, PS
Reactivity	Rat
Application Notes	F: Sections were stained using an indirect immunoperoxidase method (Ref.1). FC: Splenocytes were incubated with ER-TR7 for 30'(Ref.4). IF: Acetone or PFA fixed cells were quenched with 50mM NH4Cl for 30', blocked and permeabilized with 1.5% goat serum/0.1% saponin in PBS for 45' at RT. Incubation of ER-TR-7 in block and perm solution for 45' at RT. Specific staining was detected with a fluorescent conjugated oat anti-rat IgG (Ref.5). P: Formalin fixed paraffin sections were deparafined, hydrated in ethanol and stained with ER-TR7 for 3';at RT (Ref.3). Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Rat IgG2a
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Disclaimer	For research use only

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Protocol Information

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence