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CFLAR Antibody
SKU: orb1239384
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Description
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−Item 1 of 5
| Tested Applications | ELISA, ICC, IF, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.The immunogen is located within amino acids 180 - 230 of FLIP. |
| Target | CFLAR |
| Molecular Weight | 35 kDa and 25 kDa |
| Purification | FLIP Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | FLIP Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog
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Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution.
Immunocytochemistry staining of HeLa cells using FLIP antibody at 2 µg/mL.
Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 10 µg/mL.
Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 20 µg/mL. Red: FLIP Antibody (orb1239384) Blue: DAPI staining.
Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 2 µg/mL.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CFLAR Antibody (orb1239384)
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