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Fibronectin Antibody
SKU: orb2644350
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P |
|---|---|
| Dilution range | ELISA: order Ab without BSA for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG, kappa |
| Clone No. | FN1/3029 |
| Immunogen | A portion of amino acids 467-595 from the human protein was used as the immunogen for the Fibronectin antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IHC staining of FFPE human lung with Fibronectin antibody (clone FN1/3029). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung with Fibronectin antibody (clone FN1/3029). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Fibronectin antibody (clone FN1/3029). These results demonstrate the foremost specificity of the FN1/3029 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Fibronectin antibody (clone FN1/3029) as confirmation of integrity and purity.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Fibronectin Antibody (orb2644350)
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