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G-CSF Mouse Monoclonal Antibody

SKU: orb499770
FeaturedFeatured Product

Description

G-CSF Mouse Monoclonal Antibody

Research Area

Angiogenesis, Cancer Research, Colony Stimulating Factors (CSF), Immunology

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman, Mouse
Predicted ReactivityMouse

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG
Clone No.2C7
ImmunogenRecombinded human G-CSF protein
TargetCSF3
Molecular Weight19 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

GCSF; Colony stimulating factor 3(granulocyte); CSF 3; CSF beta; CSF3; Csfg; Filgrastim; GCSA; Granulocyte colony stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; Pluripoietin; Granulocyte Colony Stimulating Factors 3; CSF3_HUMAN.

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

G-CSF Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (G-CSF) Polyclonal Antibody, Unconjugated (orb499770) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

G-CSF Mouse Monoclonal Antibody

Sample: Lane 1: Recombinant human G-CSF protein, His, Primary: Anti-G-CSF (orb499770) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 19 kDa, Observed band size: 20 kDa.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

G-CSF Mouse Monoclonal Antibody (orb499770)

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Available Sizes

Select a size below

50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
200 μg
¥ 11,310.00
DispatchUsually dispatched within 5-10 working days
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