Galectin 13 Antibody / LGALS13

SKU: orb386036

Description

It recognizes a 32kDa protein, which is identified as homodimer of LGALS13/Galectin 13 (also known as PP13). Galectins are a family of soluble beta-galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, Galectin 13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, Galectin-13 exists as a disulfide-linked homodimer. Galectin 13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-maternal blood-spaces. Screening for Galectin 13 in maternal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the CD71 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b, kappa
Clone No.	PP13/1161
Immunogen	A human partial recombinant protein corresponding to amino acids 23-134 was used as the immunogen for the Galectin 13 antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human placenta with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 or 10mM Citrate buffer, pH6, for 10-20.

IHC testing of FFPE human spleen with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 or 10mM Citrate buffer, pH6, for 10-20.

Western blot testing of 1) human partial recombinant protein and 2) human K562 cell lysate with Galectin 13 antibody (clone PP13/1161). Expected molecular weight ~16 kDa (monomer) and ~32 kDa (dimer).

Western blot testing of human K562 cell lysate with Galectin 13 antibody (clone PP13/1161). Expected molecular weight ~16 kDa (monomer) and ~32 kDa (dimer).

SDS-PAGE analysis of purified, BSA-free Galectin 13 antibody (clone PP13/1161) as confirmation of integrity and purity.

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UniProt

Q9UHV8

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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