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Glial Fibrillary Acidic Protein Antibody / GFAP

SKU: orb1825116

Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Images & Validation

Tested ApplicationsIHC-P, WB
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.GFAP/6876
ImmunogenA recombinant partial protein sequence (within amino acids 150-250) from the human protein was used as the immunogen for the Glial Fibrillary Acidic Protein antibody.
PurificationProtein A/G affinity
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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Glial Fibrillary Acidic Protein Antibody / GFAP

IHC staining of FFPE human cerebellum tissue with Glial Fibrillary Acidic Protein antibody (clone GFAP/6876). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Glial Fibrillary Acidic Protein Antibody / GFAP

IHC staining of FFPE human brain tissue with Glial Fibrillary Acidic Protein antibody (clone GFAP/6876). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Glial Fibrillary Acidic Protein Antibody / GFAP

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using Glial Fibrillary Acidic Protein antibody (clone GFAP/6876). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol

Glial Fibrillary Acidic Protein Antibody / GFAP (orb1825116)

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Available Sizes

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20 μg
¥ 4,810.00
100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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