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Glial Fibrillary Acidic Protein Antibody / GFAP

SKU: orb606604

Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsFACS, IHC-P, WB
Dilution rangeFlow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.GFAP/2076
ImmunogenA portion of amino acids 101-200 were used as the immunogen for the Glial Fibrillary Acidic Protein antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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Glial Fibrillary Acidic Protein Antibody / GFAP

Flow cytometry testing of fixed human T98G cells with Glial Fibrillary Acidic Protein antibody (clone GFAP/2076); Red = isotype control, Blue = Glial Fibrillary Acidic Protein antibody.

Glial Fibrillary Acidic Protein Antibody / GFAP

IHC testing of FFPE human cerebellum stained with Glial Fibrillary Acidic Protein antibody (GFAP/2076). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Glial Fibrillary Acidic Protein Antibody / GFAP

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Glial Fibrillary Acidic Protein antibody (clone GFAP/2076). These results demonstrate the foremost specificity of the GFAP/2076 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Glial Fibrillary Acidic Protein Antibody / GFAP

SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone GFAP/2076) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol

Glial Fibrillary Acidic Protein Antibody / GFAP (orb606604)

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Available Sizes

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20 μg
¥ 4,810.00
100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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