Recombinant Glial Fibrillary Acidic Protein Antibody

SKU: orb606416

Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Research Area

Epigenetics

Images & Validation

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Tested Applications	FACS, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Reactivity	Human, Rat
Application Notes	The optimal dilution of the recombinant Glial Fibrillary Acidic Protein antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rASTRO/789
Immunogen	GFAP isolated from pig spinal cord was used as the immunogen for this recombinant Glial Fibrillary Acidic Protein antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of human brain lysate with recombinant Glial Fibrillary Acidic Protein antibody (clone rASTRO/789). Predicted molecular weight ~50 kDa.

IHC testing of FFPE human cerebellum tissue with recombinant Glial Fibrillary Acidic Protein antibody (clone rASTRO/789). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of fixed human T98G cells with recombinant Glial Fibrillary Acidic Protein antibody (clone rASTRO/789); Red = isotype control, Blue = recombinant Glial Fibrillary Acidic Protein antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Glial Fibrillary Acidic Protein antibody (clone rASTRO/789) as confirmation of integrity and purity.

Quick Database Links

UniProt

P14136

UniProt Details

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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