GITR Antibody (TNFRSF18)

SKU: orb2639567

Description

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF-receptor superfamily, also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-kB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

Images & Validation

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Tested Applications	FACS, IF
Dilution range	Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml
Reactivity	Mouse
Application Notes	Optimal dilution of the GITR antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG2b, lambda
Clone No.	DTA-1
Immunogen	Mouse CD25+ CD4+ T cells were used as the immunogen for the GITR antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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SDS-PAGE Analysis of Purified, BSA-Free GITR Antibody (clone DTA-1). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

O35714

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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