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GLI1 Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IHC |
|---|---|
| Dilution range | IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 1B9F8 |
| Immunogen | Purified recombinant fragment of human GLI1 (AA: 284-449) expressed in E. Coli. |
| Target | GLI1 |
| Molecular Weight | 118kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
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Immunofluorescence analysis of Hela cells using GLI1 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded ovarian cancer tissues using GLI1 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using GLI1 antibody

Western blot analysis of human recombinant protein using GLI1 antibody

Western blot analysis of HEK293 using GLI1 antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of HepG2 cells using GLI1 antibody
Protocol Information
GLI1 Antibody (orb334005)
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