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Description
Research Area
Cell Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB18221 |
| Immunogen | This GLRX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-146 amino acids from the C-terminal region of human GLRX2. Antigen Region: 120-146 aa. |
| Target | GLRX2 |
| Molecular Weight | 18052 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Glutaredoxin-2, mitochondrial, GLRX2, GRX2
Quality Guarantee
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Flow cytometric analysis of hela cells using GLRX2 Antibody (C-term T135) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain reacted with GLRX2 Antibody (C-term T135), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-GLRX2 Antibody (C-term T135) at 1:1000 dilution. Lane 1: Mouse testis tissue lysate. Lane 2: Rat testis tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_057150.2, NP_001230328.1, NP_932066.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
GLRX2 Antibody (C-term T135) (orb1929381)
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