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CSF2 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF (human Granulocyte Macrophage Colony Stimulating Factor). |
| Target | CSF2 |
| Purification | Anti-hGM-CSF specific antibody was purified by affinity chromatography employing immobilized hGM-CSF matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−GMCSF antibody [orb6090]
ICC, IF, IHC-P, WB
Guinea pig, Human, Porcine, Rat
Rabbit
Polyclonal
Unconjugated
100 μgCSF2 Antibody (Center) [orb1938788]
FC, IF, IHC-P, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μlHuman Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit [orb778165]
Human
0.32-20 ng/mL
0.113 ng/mL
96 T, 48 TDog Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit [orb779518]
Canine
7.82-500 pg/mL
3.2 pg/mL
96 T, 48 THuman Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit [orb774893]
Human
15.63-1000 pg/mL
6.5 pg/mL
96 T, 48 T

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To detect hGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human GM-CSF (orb1272524) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF.

To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
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CSF2 Antibody (orb1272525)
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