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GM-CSF Recombinant Protein
Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of human TF-1 cell proliferation.Specific Activity of 1.0 x 107 IU/ mg. |
| Target | CSF2 |
| Tag | Tag Free |
| Molecular Weight | 14.6 kD |
| Protein Sequence | Ala18-Glu144 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 5% Mannitol, pH 7.2. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human GM-CSFR Protein, hFc Tag [orb1173736]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 60.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSFR-hFc is approximately 70-130 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μgHuman GM-CSF Protein, hFc Tag [orb1173895]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSF-hFc is approximately 35-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg

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Protocol Information
GM-CSF Recombinant Protein (orb1229858)
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