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ACHE Antibody
SKU: orb19007
Description
Research Area
Epigenetics, Neuroscience
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | ACHE |
| Molecular Weight | 67.8; 54.9 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti ACHE antibody, anti YT antibody, anti acetylcholinesterase (YT blood group) antibody, anti ARACHE antibody, anti N-ACHE antibody, anti OTTHUMP00000211347 antibody, anti OTTHUMP00000211349 antibody, anti OTTHUMP00000211356 antibody, anti acetylcholinesterase; apoptosis-related acetylcholinesterase antibody
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orb19007 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of Human Brain (Cerebellum) lysate using ACHE antibody
Western blot analysis of HepG2 (A), Daudi (B) and (0.3ug/ml) HeLa (C) and Jurkat (D) cell lysate using ACHE antibody
Immunofluorescence analysis of U2OS cells using ACHE antibody
Flow cytometric analysis of HeLa cells using ACHE antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ACHE Antibody (orb19007)
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