APE1/APEX1 Antibody

SKU: orb18393

Description

Goat polyclonal antibody to APEX1

Research Area

Cancer, Cardiovascular Disease, DNA Damage, Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 4-6 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2; NP_542379.1; NP_542380.1. Recommended concentration: 0.5-2 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	APE1 / APEX1
Molecular Weight	35.6
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti APE1 antibody, anti APEX1 antibody, anti APEX antibody, anti APEX nuclease (multifunctional DNA repair enzyme) antibody, anti APE antibody, anti APX antibody, anti APEN antibody, anti HAP1 antibody, anti REF1 antibody, anti REF-1 antibody, anti AP lyase antibody, anti AP endonuclease class I antibody, anti apurinic/apyrimidinic exonuclease antibody, anti multifunctional DNA repair enzyme antibody, anti DNA-(apurinic or apyrimidinic site) lyase antibody, anti apurinic/apyrimidinic (abasic) antibody, anti APEX nuclease 1 endonuclease antibody, anti deoxyribonuclease (apurinic or apyrimidinic) antibody, anti apurinic/apyrimidinic (abasic) endonuclease antibody, anti redox factor 1 antibody

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orb18393 staining (0.5ug/ml) of A431 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot analysis of A431 (Lane 1), HeLa (Lane 2) and MCF7 (Lane 3) nuclear lysates using APEX1 antibody

Immunohistochemical staining of Human Breast using APEX1 antibody

Quick Database Links

Gene Symbol

APE1 / APEX1

NCBI Gene ID

328

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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