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CARD11 Antibody

SKU: orb18487

Description

Goat polyclonal antibody to CARD11

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration, 2-5ug/ml.WB: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetCARD11
Molecular Weight133
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti CARD11 antibody, member 11 antibody, anti BIMP3 antibody, anti CARMA1 antibody, anti MGC133069 antibody, anti card-maguk protein 1 antibody, anti bcl10-interacting maguk protein 3 antibody

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

CARD11 Antibody

Western blot analysis of HEK293 cells using CARD11 antibody

CARD11 Antibody

Immunohistochemical staining of Human Spleen using CARD11 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CARD11 Antibody (orb18487)

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Available Sizes

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100 μg
¥ 6,500.00
DispatchUsually dispatched within 5-10 working days
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