Col11a2 (aa256-268) Antibody

SKU: orb131694

Description

Goat polyclonal to Col11a2

Images & Validation

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Tested Applications	ELISA
Dilution range	ELISA: 1:64000, WB: 1 μg/ml
Predicted Reactivity	Mouse
Application Notes	Preliminary experiments gave an approx 80kDa band in lysates of cell line NIH3T3 after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 162kDa according to NP_034056.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reportedWB: Preliminary experiments gave an approx 80kDa band in lysates of cell line NIH3T3 after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 162kDa according to NP_034056.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Col11a2 (aa256-268)
Molecular Weight	162
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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Col11a2; collagen, type XI, alpha 2; RP23-222K15.2; collagen alpha-2(XI) chain; procollagen, type XI, alpha 2

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Gene Symbol

Col11a2 (aa256-268)

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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