COL4A3BP (aa662-672) Antibody

SKU: orb19732

Description

Goat polyclonal antibody to Collagen IV

Research Area

Epigenetics

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:32000, WB: 0.3-1 μg/ml
Reactivity	Mouse
Predicted Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in mouse heart lysates (calculated MW of 71.1kDa according to mouse NP_075909.1 and 60.0kDa according to human NP_112729.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	COL4A3BP (aa662-672)
Molecular Weight	70.8; 60.0
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti-Arresten antibody, anti-COL4A1 antibody, anti-COL4A1 NC1 domain antibody, anti-COL4A2 antibody, anti-COL4A3 antibody, anti-COL4A4 antibody, anti-COL4A5 antibody, anti-collagen alpha-1(IV) chain antibody, anti-Collagen IV Alpha 1 Polypeptide antibody, anti-Collagen IV Alpha 2 Polypeptide antibody, anti-Collagen Of Basement Membrane Alpha 1 Chain antibody, anti-Collagen Of Basement Membrane Alpha 2 Chain antibody, anti-Collagen Type IV Alpha 1 antibody, anti-Collagen Type IV Alpha 2 antibody, anti-Collagen Type IV Alpha 3 antibody, anti-Collagen Type IV Alpha 4 antibody, anti-Collagen Type IV Alpha 5 antibody, anti-Collagen Type IV antibody

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Western blot analysis of Mouse Heart lysate using Collagen IV antibody.

Quick Database Links

Gene Symbol

COL4A3BP (aa662-672)

NCBI Gene ID

10087

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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