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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3-5 μg/ml |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Rat |
| Application Notes |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | CSN2 / TRIP15 |
| Protein Sequence | LNSLNQAVVSKLA |
| Molecular Weight | 51.6; 52.4 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti CASB antibody, anti CSN 2 antibody, anti Csn2 antibody
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Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Thyroid Gland shows nclear and cytoplasm (3-5ug/ml) using CSN2 antibody
Western blot analysis of NIH-3T3 lysates (0.03ug/ml) using CSN2 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Gao, H. N. et al. Combination of histidine, lysine, methionine, and leucine promotes β-casein synthesis via the mechanistic target of rapamycin signaling pathway in bovine mammary epithelial cells J Dairy Sci, 100, 7696-7709 (2017)
Applications
WB
Reactivity
Bovine
Luo, Chaochao et al. Sestrin2 Negatively Regulates Casein Synthesis through the SH3BP4-mTORC1 Pathway in Response to AA Depletion or Supplementation in Cow Mammary Epithelial Cells J. Agric. Food Chem., (2019)
Applications
WB
Reactivity
Bovine
M.C. Zhang, S.G. Zhao, S.S. Wang, C.C. Luo, H.N. Gao, N. Zheng, J.Q. Wang d-Glucose and amino acid deficiency inhibits casein synthesis through JAK2/STAT5 and AMPK/mTOR signaling pathways in mammary epithelial cells of dairy cows Journal of Dairy Science, 101, 1736 (2018)
Applications
WB
Reactivity
Bovine
Jinyu Zhou 1 2 3, Shuai Lian 1 2 3, Zijian Geng 1 2 3, Yuejie Yang 4 5 6, Rui Wu 7, Jianfa Wang 8 9 10 Lactiplantibacillus plantarum promotes lactoferrin synthesis and secretion in bovine mammary epithelial cells through STAT3 and AP-1 transcription factor pathways In Vitro Cell Dev Biol Anim, (2025)
Applications
WB
Reactivity
Bovine
Dai, W. T. et al. Seryl-tRNA synthetase-mediated essential amino acids regulate β-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells J Dairy Sci, 101, 10456-10468 (2018)
Applications
WB
Reactivity
Bovine
CSN2/TRIP15 Antibody (orb18512)
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