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Goat Human Serum proteins Antibody

SKU: orb22057

Description

IgG fraction of polyclonal swine antiserum to human IgA, Fc specific. As unlabeled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user's own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non- isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5.000.

Images & Validation

Tested ApplicationsDOT, IEP
ReactivityHuman
Application Notes
Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.IE: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceDelipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
ConcentrationTotal protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
DisclaimerFor research use only

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Protocol Information

DOT
Dot Blot
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IEP
Immunoelectrophoresis
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Goat Human Serum proteins Antibody (orb22057)

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