Goat anti-IL-17B (aa77-89) Antibody

SKU: orb131701

Description

Goat polyclonal to IL17B

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	ELISA: 1:128000, WB: 1-3 μg/ml
Reactivity	Human
Application Notes	Approx 20kDa band observed in Human Ileum lysates (calculated MW of 20.4kDa according to NP_055258.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reportedWB: Approx 20kDa band observed in Human Ileum lysates (calculated MW of 20.4kDa according to NP_055258.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	IL-17B (aa77-89)
Molecular Weight	20.4
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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IL17B; interleukin 17B; IL-17B; IL-20; NIRF; ZCYTO7; cytokine Zcyto7; cytokine-like protein ZCYTO7; interleukin 20; interleukin-17 beta; interleukin-17B; interleukin-20; neuronal interleukin-17 related factor; neuronal interleukin-17-related factor

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Western blot analysis of Human Ileum lysate (35ug protein in RIPA buffer) using IL17B antibody

Quick Database Links

Gene Symbol

IL-17B (aa77-89)

NCBI Gene ID

27190

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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