Goat anti-IL12B / IL12p40 Antibody

SKU: orb19546

Description

Goat polyclonal antibody to IL12 p40

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:128000, WB: 0.3-1 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Colon shows cytoplasm staining of individual cells between columns. In paraffin embedded Human Kidney shows cytoplasm staining of epithelial cells of some DCT. Recommended concentration, 4-6ug/ml.WB: Approx 37kDa band observed in Human Liver lysates (calculated MW of 37.2kDa according to NP_002178.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	IL12B / IL12p40
Molecular Weight	37.2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti-CLMF antibody, anti-CLMF p40 antibody, anti-CLMF2 antibody, anti-IL 12 subunit p40 antibody, anti-IL 12B antibody, anti-IL-12 subunit p40 antibody, anti-IL-12B antibody, anti-IL12 subunit p40 antibody, anti-IL12B antibody, anti-interleukin 12 beta chain antibody, anti-Interleukin 12 p40 antibody, anti-Interleukin 12 subunit beta antibody, anti-Interleukin 12B antibody, anti-Interleukin-12 subunit beta antibody, anti-NK cell stimulatory factor chain 2 antibody, anti-NKSF antibody, anti-NKSF2 antibody, anti-p40 antibody, anti-IL12 p40 antiody, anti-IL 12 p40 antiody, anti-IL 12p40 antiody

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orb19546 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19546 (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 9, HRP-staining.

Western blot analysis of Mouse Liver (lane 1) and Testes (lane 2) and Rat Brain (lane 3) lysate using IL12 p40 antibody

Quick Database Links

Gene Symbol

IL12B / IL12p40

NCBI Gene ID

3593

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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